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M9490408.TXT
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1994-09-19
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Document 0408
DOCN M9490408
TI Analysis of a rape case by direct sequencing of the human
immunodeficiency virus type 1 pol and gag genes.
DT 9411
AU Albert J; Wahlberg J; Leitner T; Escanilla D; Uhlen M; Department of
Clinical Virology, Swedish Institute for Infectious; Disease Control,
Karolinska Institute, Stockholm.
SO J Virol. 1994 Sep;68(9):5918-24. Unique Identifier : AIDSLINE
MED/94335109
AB Transmission of human immunodeficiency virus type 1 (HIV-1) from a male
accused of rape and deliberate transmission of HIV-1 was investigated by
sequencing of the HIV-1 pol and gag genes from virus obtained from the
male and from the female victim. Parts of the reverse transcriptase and
p17gag genes were amplified and directly sequenced from uncultured
peripheral blood mononuclear cells. The sequences were compared with
sequences from 21 unrelated HIV-1-infected controls from the same
geographic area (Stockholm, Sweden). Bootstrap analysis of phylogenetic
trees demonstrated that the sequences from the female were significantly
more closely related to the sequences from the male than to sequences
from the controls. Furthermore, we found that the male and female shared
two distinct genetic variants of HIV-1. In p17gag the major variant had
an unusual, out-of-frame deletion of 3 nucleotides which the minor
variant lacked. These results indicated that the male had transmitted
more than one infectious unit to the female. From this study we
concluded that it was highly likely that the HIV-1 strains carried by
the male and female were closely epidemiologically linked.
DE Base Sequence Comparative Study Drug Resistance, Microbial DNA
Primers/CHEMISTRY Female *Genes, gag *Genes, pol Human
HIV-1/*GENETICS Male Molecular Sequence Data Phylogeny *Rape
Reverse Transcriptase/CHEMISTRY Sequence Alignment Sequence Homology,
Amino Acid Support, Non-U.S. Gov't Zidovudine/PHARMACOLOGY JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).